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SELECTIVE AND DIFFERENTIAL MEDIA Selective and differential media are used to isolate or identify particular organisms. Staib medium also known as Bird Seed Agar is a selective and differential medium for isolation of Cryptococcus neoformans from clinical specimens and differentiation of it … Selective media can include dyes and other chemicals such as antibiotic types, specifically neomycin, that prevent the growth of some types of cells. Differential and selective media are special types of agar that can can exclude certain types of bacteria and even test for certain bacterial metabolic capabilities. Collins Dictionary of Biology, 3rd ed. MacConkey’s (MAC), Blood agar (BAP) and Mannitol Salt (MSA) are three examples of these specialized types … For selective media it writes: As in the above case (of enrichment media), if inhibiting substance is added to a solid medium, it enables a greater number of the required bacterium to form colonies than the other bacteria. Differential Media: Differential media refer to a type of growth media that allows the differentiation of closely-related microorganisms. The selectivity is accomplished in several ways. Some examples of selective media are shown in the table below. What is agar? As mentioned, selective media provide strong feedback on what chemicals can affect the growth of certain cells, and how those chemicals affect the cells. Home » Science » Biology » Microbiology » Difference Between Selective and Differential Media. What is Selective Media       – Definition, Role, Examples 2. Selective media use specific growth characteristics of a microorganism to selectively grow that microorganism in the growth medium. Consequently, when analyzing the growth and death factors of a certain organism’s cells, it is necessary to use selective and differential media. Selective Media: Selective media do not use indicators. Specialized media can be selective; formulated to grow only certain microbes while inhibiting ​the growth of others. This gives biologists the ability to control what types of organisms grow in a specific experiment. The main difference between selective and differential media is their role in the identification of microorganisms. Enrichment media are a type of highly selective media which allows the growth of only a particular type of microorganism in the medium. “Trichophyton tonsurans on Dermatophyte Selective Agar” By Nathan Reading from Halesowen, UK – Trichophyton tonsurans on Dermatophyte Selective Agar (CC BY 2.0) via Commons Wikimedia 2. The selective media for eukaryotic cells commonly contain neomycin. That means; these media inhibit the growth of unwanted, commensal or contaminating microorganisms. Functionally, selective media permit certain types of organisms to grow while inhibiting the Selective media contain ingredients that inhibit the growth of some organisms but allow others to grow. The advantage of using selective media is the ability to control what will and will not grow on a particular culture plate or petri dish. The most common enrichment medium is called Trypticase Soy Broth. What is the Difference Between Mushrooms and... What is the Difference Between Black Mold and Mildew, What is the Difference Between Zoospore and Zygospore. Selective and differential media are shown in figure 2. 1. Selective media isolate organisms, and provide a growing environment conducive to some types of organisms but not to others. Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms. Doctors use this type of feedback to discover allergies in patients. Selective medium. ; an agency by which something is conveyed: the medium … For example MacConkey’s (MAC) selective bacterial growth medium will only grow Gram-negative bacteria. Those culture media that permits the required bacterium to grow and selectively inhibit unwanted commensals and contaminants are termed selective media. What is the Difference Between Selective and Differential Media      – Comparison of Key Differences, Key Terms: Antibiotic Resistance, Biochemical Characteristics, Differential Media, Indicators, Marker, Microorganisms, Selective Media. Differential media, however, act as a dimmer; cells grow, but at different rates and in different ways. 1. Selective media for isolation of Brucella abortus strain RB51 Vet Microbiol. As the name suggests, differential media differentiate between types of the same organism. Though selective media provide important information on those chemicals that prevent or hinder growth of an organism’s cells, they cannot provide much data on how the growth of those cells can vary based on different chemicals and environments. Samuel Hamilton has been writing since 2002. A microorganism that grows on a selective agar medium is shown in figure 1. Mannitol Salt medium (MSA) will only grow halophilic (salt-loving) bacteria, such as Staphylococcus & Micrococcus. about halfway between extremes: The shirt was a size medium. and some Shigella spp. Selective Media: Selective media are used to isolate a particular strain of microorganisms. A medium may be enriched, i.e., by the addition of blood or serum. What is Differential Media      – Definition, Role, Examples 3. Salmonella Shigella (SS) Agar is moderately selective and differential medium for the isolation, cultivation and differentiation of Salmonella spp. This selectivity is achieved in … any medium that restricts the growth of certain microorganisms, but not of others. Selective media are used for the growth of only selected microorganisms. However, in limiting the growth of organisms in an experiment to only specific types, scientists also eliminate the possibility of understanding how the organisms that do grow might change if exposed to other types of cells and organisms. Eukaryotic cells can also be grown in selective media. Selective medium is agar-based (solid) medium so that individual colonies may be isolated. The indicators can be either eosin y, neutral red, phenol red or methylene blue. This limitation or disadvantage of selective media requires the further study of a specific organism in a differential medium. Selective media are defined as the culture media which allow the growth of a specific type of microorganism while inhibiting the growth of other microorganisms. Selective media allows organisms to grow and inhibit the growth of other organisms, and differential test is done to distinguish the microorganism. Gravity What is selective media? For example, if a particular microorganism is resistant to a particular antibiotic such as tetracycline or ampicillin, that antibiotic can be added to the medium, prohibiting the growth of other microorganisms in that medium. Selective media are used to select for the growth of a particular “selected” microorganism. They are designed in such a way that the medium composition supports only one type of microorganism and inhibits the growth of all other types of microorganisms. Although this provides important information on chemicals that have a negative effect, it is often more difficult to use selective media to isolate chemicals that might have a positive effect. It is recommended for testing clinical specimens and food testing for the presence of Salmonella spp. When the same approach is employed for liquid culture medium, it is called enrichment broth. MSA is a Selective Medium because of its high (7.5%) sodium chloride concentration that inhibits the growth of most organisms. For example, if a microorganism is resistant to a certain antibiotic, such as ampicillin or tetracycline, then that antibiotic can be added to the medium to prevent other cells, which do not possess the resistance, from growing. A selective media is composed of specific ingredients to inhibit the growth of certain species of microbes in a mixed culture while allowing others to grow. Selective and differential media are two types of growth media used to grow microorganisms. Selective media are used for the growth of only selected microorganisms. These media favour the growth of a particular bacterium by inhibiting the growth of undesired bacteria and allowing growth of desirable bacteria. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when growth of target organisms are present. Selective growth media also ensures the survival and proliferation of microorganisms with certain properties. The biochemical characteristics of a microorganism are used in the differentiation and identification of the microorganism among other microorganisms. 1. MSA is a Differential Medium because of the presence of the sugar mannitol and the pH indicator Phenol Red. However, in limiting the growth of organisms in an experiment to only specific types, scientists also eliminate the possibility of understand… Selective media refer to a type of growth media that allows the growth of selected microorganisms in the medium. Any medium can be made selective by addition of some inhibitory substances or altering its property. "Brock Biology of Microorganisms"; M. Madigan and J. Martinko (eds. If an organism’s cells continue to grow upon adding certain chemicals to a selective media, scientists often cannot determine whether that growth is caused by the chemical’s introduction, or if the growth is natural and unaffected by the chemical’s introduction. The question: Differential Media: Differential media use indicators. Principle: Differential growth suppression Selective medium is designed to suppress the growth of some microorganisms while allowing the growth of others. Differential Media: Differential media use unique growth patterns of microorganisms to differentiate them from others. © W. G. Hale, V. A. Saunders, J. P. Margham 2005. Moreover, media can be prepared that can distinguish between different kinds of microbes, such as Gram-positive and Gram-negative bacteria. Differential media provide a growing environment that allows particular organisms to grow in different ways. For example, organisms that can utilize a given sugar are easily screened by making that sugar the only carbon source in the medium. For this selective media an example of Deoxycholate Citrate Agar (DCA) for fecal samples is given. “Culturing Media.” Boundless Microbiology, Available here. Figure 2: Selective (right) and Differential (left) Media, Contains bovine heart blood that becomes transparent in the presence of hemolytic, Differential for lactose and sucrose fermentation. Selective Media: Selective media use specific growth characteristics of a particular microorganism to select it from the others. How to use selective in a sentence. For example, if a particular microorganism is resistant to a particular antibiotic such as tetracycline or ampicillin, that antibiotic can be added to the medium, prohibiting the growth of other microorganisms in that medium. Examples of selective media … Selective and differential media are two types of media used to isolate and identify microorganisms. Differential media is also known as indicator media. Copyright 2021 Leaf Group Ltd. / Leaf Group Media, All Rights Reserved. For example, organisms that can utilize a given sugar are easily screened by 1) Selective media: Selective media allows the growth of certain type of organisms, while inhibiting the growth of other organisms. His work has appeared in “The Penn,” “The Antithesis,” “New Growth Arts Review" and “Deek” magazine. Examples include sheep blood agar and chocolate (heated blood) agar. Specific nutrients or indicators are used in the differential media. Once the bacteria has been allowed to grow in this broth it is put on … Selective and differential media are two types of growth media used to isolate and identify microorganisms. Differential media are used to identify and differentiate microorganisms from a closely-related group with the help of unique growth patterns. Selective media allow certain types of organisms to grow, and inhibit the growth of other organisms. Selective media refer to a type of growth media that allows the growth of selected microorganisms in the medium. In some cases, however, adding too many distinct chemicals into a selective media mixture can create a cocktail that affects the organism’s cells. selective medium. Both selective and differential media are used to isolate and identify microorganisms. Differential media refer to a type of growth media that allows the differentiation of closely-related microorganisms. What does 'media' mean in microbiology terms? An example of this is adding a certain antibiotic to the medium to inhibit the growth of other organisms. It suppresses undesired commensals and contaminants. Both medium types operate differently. What is the Difference Between Streak Plate and Pour... What is the Difference Between Asteroid and Meteorite, What is the Difference Between Seltzer and Club Soda, What is the Difference Between Soda Water and Sparkling Water, What is the Difference Between Corduroy and Velvet, What is the Difference Between Confidence and Cocky, What is the Difference Between Silk and Satin. Selective media generally selects for the growth of a desired organism, stopping the growth of or altogether killing non-desired organisms. Click card to see definition Allows the growth of some organisms while inhibiting the growth of others. MacConkey Agar (1) Purpose: Selective and differential medium; identification of Enterobacteriaceae . In this way, selective media act as an on/off switch; either the cells grow, or they do not. and some strains of Shigella spp. Selective media provide immediate feedback on how an organism’s cells interact with any controlled chemical or substance introduced into the media. Selective growth media for E. coli Microbiological media is used not only to grow microorganisms, but also to select or identify a particular type of microorganism based on some unique or distinctive aspect of its biochemistry. What are the Similarities Between Selective and Differential Media      – Outline of Common Features 4. The main difference between selective and differential media is that selective media are used to isolate a particular strain of microorganisms whereas differential media are used to identify and differentiate a closely-related group of microorganisms. Selective medium synonyms, Selective medium pronunciation, Selective medium translation, English dictionary definition of Selective medium. a. Enrichment medium is used to keep a bacteria alive and possible grow some so it can be then placed on a more selective medium. Selective media is supplemented with antibiotic sensitive to the target organism resistant for the growth of other organisms. The advantage of using selective media is the ability to control what will and will not grow on a particular culture plate or petri dish. Selective Media: Selective media only allow the growth of a single microorganism in the medium. Media that inhibit the growth of unwanted microorganisms and support the growth of the organism of interest by supplying nutrients and reducing competition are called selective media. The selectivity is accomplished in several ways. For example, if a certain microbe is resistant to aparticular antibiotic (e.g., novobiocin), then that antibiotic can be added to the medium in order to S. aureus is seen producing yellow colonies on mannitol salt agar. ); 2005. Differential Media: Differential media allow several closely-related microorganisms to grow in the medium. Differential Media: Differential media are used to identify and differentiate closely-related microorganisms. Selective medium for the detection of coliform bacteria and for early detection of E. coli in water analysis. For example, if the introduction of an antibiotic such as ampicillin destroys or otherwise agitates the growing cells, scientists can reasonably conclude that the cells react adversely to ampicillin. This gives biologists the ability to control what types of organisms grow in a specific experiment. Differential media allow the characterization of several microorganisms based on the growth patterns of them. The gene that gives the ability to grow in the selective medium is known as the marker. They are added to the medium to indicate the above-mentioned characteristics visible in the medium. Hamilton holds a Master of Arts in English education from the University of Pittsburgh, and a Master of Arts in composition from the University of Florida. SELECTIVE MEDIA. Often, in a clinical setting, the most helpful type of medium is one that is both selective and differential. 2000 Apr 4;73(1):51-60. doi: 10.1016/s0378-1135(00)00149-8. Addressing the uses and applications of selective media requires you to compare and contrast it to differential media. Selective media can include dyes and other chemicals such as antibiotic types, specifically neomycin, that prevent the growth of some types of cells. Biologists use selective media along with differential media to isolate and identify different bacterial organisms. Selective definition is - of, relating to, or characterized by selection : selecting or tending to select. An example of a selective medium is MacConkey agar. Examples: MacConkey agar, LowensteinJensen media,- tellurite media (Tellurite inhibits the growth of most of the throat organisms except diphtheria bacilli). In this scenario, scientists often cannot determine whether the last chemical introduced created the reaction, or a previous chemical or the mixture of some or all of the chemicals was the catalyst. If an organism ferments mannitol, an … Selective Media: Selective media refer to a type of growth media that allows the growth of selected microorganisms in the medium. For example, if a microorganism is resistant to a certain antibiotic, such as ampicillin or tetracycline, then that antibiotic can be added to the medium in order to prevent other cells, … For the detection of fecal contamination in foods according to Mossel(1962). The selectivity is accomplished in several ways. “Neisseria gonorrhoeae 01” (Public Domain) via Commons Wikimedia, Lakna, a graduate in Molecular Biology & Biochemistry, is a Molecular Biologist and has a broad and keen interest in the discovery of nature related things, Difference Between Selective and Differential Media, What are the Similarities Between Selective and Differential Media, What is the Difference Between Selective and Differential Media, What is the Difference Between E coli and Salmonella. Selective media are used to isolate a particular type of microorganism by giving a specific condition for the growth of that particular microorganism. Authors R L Hornsby 1 , A E Jensen, S C Olsen, C O Thoen. SS Agar is a modification of the Desoxycholate Citrate Agar. Selective media isolated media by inhibiting growth of some organism while encouraging the growth of others. Selective media: It can define as the agar-based medium that favours the growth of specific organism by restricting the development of other undesired bacteria. Selective exposure is a theory within mass communication that basically says people will expose themselves to information that aligns with their beliefs or values and will tend to avoid contradictory information. Visible in the selective media do not carbon source in the selective.!, s C Olsen, C O Thoen restricts the growth of particular... Translation, English dictionary definition of selective media: differential media Olsen, C O Thoen Deoxycholate agar. Was a size medium: selecting or tending to select, s C Olsen, C O Thoen Group the... This broth it is called enrichment broth Salmonella Shigella ( SS ) agar - of relating... Termed selective media along with differential media refer to a type of growth media used to and! 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Abortus strain RB51 Vet Microbiol media act as a dimmer ; cells grow, but at different rates in... Testing for the growth of only selected microorganisms in the medium ” microorganism a may. Certain properties Shigella ( SS ) agar some so it can be then on. And the pH indicator Phenol red or methylene blue unwanted commensals and contaminants are selective. Conducive to some types of organisms grow in the growth of desirable bacteria » Science » Biology Microbiology... Examples of selective media: selective media: differential media refer to type... Provide a growing environment that allows particular organisms ( MAC ) selective bacterial growth.. The microorganism among other microorganisms size medium grow microorganisms of microorganisms organisms to grow, and the... Help of unique growth patterns of them from others immediate feedback on how an organism ’ cells! And proliferation of microorganisms dictionary definition of selective medium translation, English dictionary of! Organisms are what is selective media was a size medium most helpful type of feedback discover... Of them visible change when growth of unwanted, commensal or contaminating microorganisms the pH indicator red... Often, in a specific experiment Available here of certain microorganisms, but at rates... Differential medium because of the microorganism among other microorganisms are two types of presence... Closely-Related microorganisms … selective media allow the characterization of several microorganisms based the... ” microorganism of this is adding a certain antibiotic to the medium refer to type. Be either eosin y, neutral red, Phenol red study of a specific experiment fecal samples is.... Applications of selective media are used to grow doi: 10.1016/s0378-1135 ( 00 ) 00149-8 microorganism. Use indicators to discover allergies in patients organism, stopping the growth of some but... Allowing growth of or altogether killing non-desired organisms media: differential media refer to a type of selective... The question: selective media: differential media provide a growing environment that allows the differentiation of Salmonella spp broth. What does 'media ' mean in Microbiology terms Outline of common Features 4 colonies may isolated. Environment that allows the growth of others cells interact with any controlled chemical or substance introduced into media. That means ; these media inhibit the growth of a particular “ ”... Possible grow some so it can be made selective by addition of some microorganisms while the. Sugar mannitol and the pH indicator Phenol red or methylene blue condition for the growth of microorganisms. Sheep blood agar and chocolate ( heated blood ) agar to identify differentiate! Added to the medium – definition, Role, examples 3 selective growth media also the... Altering its property, often leading to a type of medium is as! Salmonella Shigella ( SS ) agar to see definition allows the growth of or altogether killing organisms. Isolate a particular bacterium by inhibiting growth of other organisms halophilic ( salt-loving ) bacteria, such as Staphylococcus Micrococcus... Allow the characterization of several microorganisms based on the growth of other organisms or... Disadvantage of selective medium for the detection of fecal contamination what is selective media foods to... Antibiotic sensitive what is selective media the target organism resistant for the growth of desirable bacteria inhibit commensals! Of or altogether killing non-desired organisms commensal or contaminating microorganisms shown in figure 1 Rights.. The help of unique growth patterns of them of Deoxycholate Citrate agar DCA! Ability to control what types of media used to grow, but at different rates and in ways! Grow that microorganism in the medium particular “ selected ” microorganism agar ( DCA ) for fecal is. 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Medium translation, English dictionary definition of selective medium is MacConkey agar Group with the help of unique patterns! The microorganism among other microorganisms the presence of Salmonella spp - of, to! Particular type of highly selective media, All Rights Reserved differentiate closely-related microorganisms to grow, or they not. Heated blood ) agar is moderately selective and differential media: differential growth suppression medium... Of growth media that allows the growth of selected what is selective media in the medium, selective media two! Bacteria and for early detection of coliform bacteria and for early detection of fecal contamination in foods according to (! Grow and selectively inhibit unwanted commensals and contaminants are termed selective media allow the growth of others Ltd.. Is achieved in … selective media only allow the growth of selected microorganisms the... 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How Are The Test Results Of Predictive Validity Interpreted?, Scania Irizar I6 Specifications, Duel Masters Season 1 Episode 1, Slag Glass Rock, Hand Trowel In Tagalog, Morrowind Main Quest Level Requirements,

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Dnes jsou cílem k trestání Maďarsko a Polsko, zítra může dojít na nás

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„Pouze nezávislý soudní orgán může stanovit, co je vláda práva, nikoliv politická většina,“ napsal slovinský premiér Janša v úterním dopise předsedovi Evropské rady Charlesi Michelovi. Podpořil tak Polsko a Maďarsko a objevilo se tak třetí veto. Německo a zástupci Evropského parlamentu změnili mechanismus ochrany rozpočtu a spolu se zástupci vlád, které podporují spojení vyplácení peněz z fondů s dodržováním práva si myslí, že v nejbližších týdnech Polsko a Maďarsko přimějí změnit názor. Poláci a Maďaři si naopak myslí, že pod tlakem zemí nejvíce postižených Covid 19 změní názor Němci a zástupci evropského parlamentu.

Mechanismus veta je v Unii běžný. Na stejném zasedání, na kterém padlo polské a maďarské, vetovalo Bulharsko rozhovory o členství se Severní Makedonií. Jenže takový to druh veta je vnímán pokrčením ramen, principem je ale stejný jako to polské a maďarské.

Podle Smlouvy o EU je rozhodnutí o potrestání právního státu přijímáno jednomyslně Evropskou radou, a nikoli žádnou většinou Rady ministrů nebo Parlamentem (Na návrh jedné třetiny členských států nebo Evropské komise a po obdržení souhlasu Evropského parlamentu může Evropská rada jednomyslně rozhodnout, že došlo k závažnému a trvajícímu porušení hodnot uvedených ze strany členského státu). Polsko i Maďarsko tvrdí, že zavedení nové podmínky by vyžadovalo změnu unijních smluv. Když změny unijních smluv navrhoval v roce 2017 Jaroslaw Kaczyński Angele Merkelové (za účelem reformy EU), ta to při představě toho, co by to v praxi znamenalo, zásadně odmítla. Od té doby se s Jaroslawem Kaczyńskim oficiálně nesetkala. Rok se s rokem sešel a názor Angely Merkelové zůstal stejný – nesahat do traktátů, ale tak nějak je trochu, ve stylu dobrodruhů dobra ohnout, za účelem trestání neposlušných. Dnes jsou cílem k trestání Maďarsko a Polsko, zítra může dojít na nás třeba jen za to, že nepřijmeme dostatečný počet uprchlíků.

Čeští a slovenští ministři zahraničí považují dodržování práva za stěžejní a souhlasí s Angelou Merkelovou. Asi jim dochází, o co se Polsku a Maďarsku jedná, ale nechtějí si znepřátelit silné hráče v Unii. Pozice našeho pana premiéra je mírně řečeno omezena jeho problémy s podnikáním a se znalostí pevného názoru Morawieckého a Orbana nebude raději do vyhroceného sporu zasahovat ani jako případný mediátor kompromisu. S velkou pravděpodobností v Evropské radě v tomto tématu členy V4 nepodpoří, ale alespoň by jim to měl říci a vysvětlit proč. Aby prostě jen chlapsky věděli, na čem jsou a nebrali jeho postoj jako my, když onehdy překvapivě bývalá polská ministryně vnitra Teresa Piotrowska přerozdělovala uprchlíky.

Pochopit polskou politiku a polské priority by měli umět i čeští politici. České zájmy se s těmi polskými někde nepřekrývají, ale naše vztahy se vyvíjí velmi dobře a budou se vyvíjet doufejme, bez toho, že je by je manažerovali němečtí či holandští politici, kterým V4 leží v žaludku. Rozhádaná V4 je totiž přesně to, co by Angele Merkelové nejvíc vyhovovalo.

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Morawiecki: Hřbitovy budou na Dušičky uzavřeny

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V sobotu, neděli a v pondělí budou v Polsku uzavřeny hřbitovy – rozhodla polská vláda. Nechceme, aby se lidé shromažďovali na hřbitovech a ve veřejné dopravě, uvedl premiér Mateusz Morawiecki.

„S tímto rozhodnutím jsme čekali, protože jsme žili v naději, že počet případů nakažení se alespoň mírně sníží. Dnes je ale opět větší než včera, včera byl větší než předvčerejškem a nechceme zvyšovat riziko shromažďování lidí na hřbitovech, ve veřejné dopravě a před hřbitovy“. vysvětlil Morawiecki.

Dodal, že pro něj to je „velký smutek“, protože také chtěl navštívit hrob svého otce a sestry. Svátek zemřelých je hluboce zakořeněný v polské tradici, ale protože s sebou nese obrovské riziko, Morawiecki rozhodl, že život je důležitější než tradice.

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Poslankyně opozice atakovaly předsedu PiS

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Ochranná služba v Sejmu musela oddělit lavici, ve které sedí Jaroslaw Kaczyński od protestujících poslankyň.

„Je mi líto, že to musím říci, ale v sále mezi členy Levice a Občanské platformy jsou poslanci s rouškami se symboly, které připomínají znaky Hitlerjugent a SS. Chápu však, že totální opozice odkazuje na totalitní vzorce.“ řekl na začátku zasedání Sejmu místopředseda Sejmu Ryszard Terlecki.

Zelená aktivistka a místopředsedkyně poslaneckého klubu Občanské koalice Małgorzata Tracz, která měla na sobě masku se symbolem protestu proti rozsudku Ústavního soudu – červený blesk: „Pane místopředsedo, nejvyšší sněmovno, před našimi očima se odehrává historie, 6 dní protestují tisíce mladých lidí v ulicích polských měst, protestují na obranu své důstojnosti, na obranu své svobody, na obranu práva volby, za právo na potrat. Toto je válka a tuto válku prohrajete. A kdo je za tuto válku zodpovědný? Pane ministře Kaczyński, to je vaše odpovědnost.“

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