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total chlorophyll estimation by spectrophotometer

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2021-01-06T05:27:49-08:00 2021-01-06T05:27:49-08:00 However, if the samples could not be analysed, immediately, the samples were stored in the refrigeration, below 4 °C. According to PCA analysis, temperature, salinity, total dissolved solids, ammonia nitrogen, nitrite, and phosphate were the most influential parameters contributing to the water quality fluctuations in the PRE. phosphorus (TP), water flow, light, catchment area, water depth, weather, and other physical factors. 16 0 obj endobj Changes in absorbance between 440 and 410 nm cannot be used for the estimation of phaeophytin. To calculate the chlorophyll-a. Chlorophyll-a concentration is an important, indicator for detecting microalgae in water bodies. Chlorophyll concentrations are measured by spectrophotometry as: [ Chl a] = 13.43 ( A 663.8 – A 750) – 3.47 ( A 646.8 – A 750) ( nmol mL – 1) [ Chl b] = 22.90 ( A 646.8 – A 750) – 5.38 ( A 663.8 – A 750) ( nmol mL – 1) here [Chl a] and [Chl b] are molar concentrations of chlorophyll a and chlorophyll b, respectively. Chlorophyll extraction by three solvents in three aquatic plants Total chlorophylls of duckweed extracted by 80% acetone, 90% acetone, and 95% ethanol were 0.648 * 0.012 mg g-', 0.860 * 0.008 mg g-', and 0.745 * 0.018 mg g-', respectively. Densities of microbes and meiofauna were highest in July, and decreased in winter. The samples were tested using 90% acetone method by using spectrophotometer (model HITACHI U-1900) to record the absorbance values with selected wavelengths namely … Chlorophyll can easily be quantified with a spectrophotometer based on the Beer-Lambert Law and the extinction coefficient for chlorophyll. In the visible spectrum, microalgae recorded the highest reflectance value in the range from 550 to 560 nm (green region) and around 700nm (red region). In this stu, acetone was used to measure the chlorophyll-a, concentration with some selected wavelengths. Figure 1: Absorbance spectra of chlorophylls a and b. Ħ?| i��Ҕ@L��NhRWX?�������%`� (2]b! Both samples were at a concentration of 1mg/l. The, determination from freshwater microalgae is, spectrophotometer analysis at selected wavelength of, Absorption spectrophotometer is a technique used in. endobj application/pdf In-vitro digestibility was higher in the rainy period (P < 0.05), although in the dry season, digestibility at a cutting height of 25 cm was greater. Regression analysis showed algal abundance per unit phosphorus increasing in the sequence: rivers < impoundments < natural lakes, which paralleled intergroup differences in residence time. The biodiesel from safflower seed oil or algal oil in itself is not significantly different from biodiesel produced from vegetable oils. The 44 habitat, We studied the vertical distribution of microbes and meiofauna in natural hydrocarbon seep sediments to determine if there was a relationship between profiles of benthic trophic structure and the unique biogeochemical conditions present at the seep. UV-260 spectrophotometer. The Spectral Determination of Chlorophylls a and b, as well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution - ScienceDirect. The lipid and fatty acid contents of microalgae vary in accordance with culture conditions. <>/ProcSet[/PDF/Text/ImageB]/XObject<>>>/Type/Page>> endobj We investigated the content of carotenoids and chlorophylls of leaves from Pallenis spinosa (P. spinosa), as well as their antioxidant activity and mineral composition then, we optimized the solvent extraction for the recovery of total carotenoids and chlorophylls using spectrophotometric method. The presence of phaeophytin (Ph) a not only interferes with estimates of Chl a but also with Chl b and c determinations. The spectrophotometer with, 90% acetone method successfully determined the, microscopic organisms. In the rainy period, forage should be collected at a height of 25 cm and after a regrowth period of 60 days, while in the dry period, the ideal cutting height is 50 cm, at a regrowth age of 60 days. The chlorophyll a and carotenoid content was calculated using the following modified equations (Jeffrey and Humphrey, 1975; ... Aqueous acetone is the most widely used solvent because it allows better stability for CHLA compared to methanol. endobj 20 0 obj In, number of so-called primary absorbing pigments. The Pearson correlation coefficient was calculated to elucidate the correlation among the ecological parameters in the PRE during the dry season. Conclusions Preferred food organisms consisted of filamentous cyanobacteria, mainly Arthrospira, as well as benthic diatoms. Therefore, the chlorophyll in algae is stable, In addition, the measurement of algal biomass is, important in aquatic studies and is commonl, by determining the chlorophyll-a concentration. producer in the food web in freshwater and ocean. 8 0 obj Water temperature is, one of factors that potentially influence the growth of the, microalgae. In situ spectral signatures of 19 mangrove species were measured to investigate whether mangrove species could be discriminated through spectral reflectance data. dalam sistem fotobioreaktor setara dengan penambahan kelimpahan biomassa mikroalgae. The extracts were then centrifuged for 5 min in a glass tube and subsequently assayed by a UV–Visible spectrophotometer (UV-1280, Shimadzu, Japan). The grinding tube is kept on ice to minimize heat from friction during extraction. Silat was chosen as it is widely practiced by Malaysian-Indonesian and there are many branches of silat practice by them. Microalgae, or also known, protein. endstream = A480+ (0.114 × A663) ─ (0.638 × A 645) Where, A = Absorbance at respective wave length 2.7 Estimation of Fucoxanthin This is because microalgae are primary producer in the food web in freshwater and ocean. The concentrations of chlorophyll-a in the, microalgae were measured by spectrophotometer using, Prior to spectrophotometric analysis, all microalgae, rpm in centrifuge machine. at various dehydration levels, Antioxidant activity, carotenoids, chlorophylls and mineral composition from leaves of Pallenis spinosa: an Algerian medicinal plant, Effects of different regrowth ages and cutting heights on biomass production, bromatological composition and in vitro digestibility of Guazuma ulmifolia foliage, Analysis of Chlorophyll Concentration in Potato Crop by Coupling Continuous Wavelet Transform and Spectral Variable Optimization, In-site chlorophyll-a fluorometer based on lock-in amplifier, Preliminary study: Spectral reflectance properties of microalgae in freshwater, A Large-Scale Comparison of Factors Influencing Phytoplankton Abundance in Rivers, Lakes, and Impoundments, Extraction of Chlorophyll a From Freshwater Phytoplankton for Spectrophotometric Analysis, Comparison of different methods for chlorophyll and phaeopigment, Qualifying of safflower and algae for energy, Effects of Environmental Factors on the Seasonally Change of Chlorophyll-a in Eutrophic Plateau Lake Dianchi, China, Physiological Plant Ecology: Ecophysiology and Stress of Function Groups, Inversion modeling of chlorophyll-a concentration in water by multi-angle polarized reflection, The use of acetone and methanol in the estimation of chlorophyll in the presence of phaeophytin, A comparison between two methods of extracting chlorophyll-a from different phytoplankton samples, Study of Metaphysical Behaviour With Different Dynamic Motion Using 3D Motion Capture Camera, "Jurus silat" estimation power using MOCAP, APPLICATION OF AN OVERLAY TECHNIQUE TO DETERMINE A POTENTIAL GEOTHERMAL, Algal Cultivation and Hydrothermal Gasification: Biomass and Energy Production, Food algae for Lesser Flamingos: a stocktaking. Eight minerals have been detected (Mg, Ca, P, Fe, Mn, Zn, Cu and Cr), Mg and Ca being the predominant ones (6479.32 ± 48.33 and 3851.88 ± 130.63 mg/Kg, respectively). The chlorophyll extracted by 90% acetone was higher than that extracted by The absorbance maximum for chlorophyll a was determined to be 662.6nm and for chlorophyll b, 645.6nm. The. Chlorophyll-a (Chl-a) concentration is an important indicator for detecting lake waters. QUANTITATIVE ESTIMATION OF TOTAL CHLOROPHYLL AND CAROTENOID CONTENT IN OREOPANAX XALEPENSIS Kishan Kshetrimayum1, Renuka Devi N1, ... spectrophotometry INTRODUCTION Manipur located in the Indo-Burma biodiversity hotspot region is famous for its rich flora and fauna. Algal abundance per unit phosphorus did not differ significantly among these three system types when systems with similar residence times were compared. Guazuma ulmifolia (G. ulmifolia) is a tropical tree species with potential in ruminant feeding. The chlorophyll-a concentration in microalgae depended on several factors such as amount of sunlight available, total phosphorus (TP), water flow, light, catchment area, water depth, weather, and other physical factors. Of the, three wavelengths, the chlorophyll-a concentration of, highest absorbance. Abstract In this study, the samples of microalgae were collected from Tasik Harapan and Tasik Aman, USM Penang, Malaysia. Cluster analysis grouped the ecological parameters into two different clusters as follows: cluster 1 (salinity, TDS, temperature and NH4+), cluster 2 (pH, DO, NO3-, NO2-, PO43- and chlorophyll a). 1) A 662 , 2) A 663 , and 3) A 645 . Table-1 Equations to determine concentrations (µg/ml) of chlorophyll a (Ch-a), chlorophyll b (Ch-b) and total carotenoids (C x+c) by different extractant solvents in spectrophotometer 12,14,18 Solvents Equations/Formula 80% Acetone Capability of cell on sequestering ofCO2 increased amounting of 102 – 167.2 % per day.Keywords: Chlorella sp, carbondioxide, sequestration, microalgae abundanceAbstrak Chlorella sp. Potential important wave bands that can be used to discriminate mangrove species were identified by using linear discriminant analysis (LDA) (at 99.95% level). The reflectance data was analysed using one-way ANOVA to identify bands that exhibit significant difference (at 99.99% level) across the mangrove species. endobj A circular piece was cut from each leaf after NIR and color estimated for total chlorophyll content extraction, using a mortar to grand the leaf into powder and extracting with 100% acetone. All population sizes decreased with increasing depth in the sediment. For. Density and abundance of nematodes, bacteria cell counts, and bacterial biomass were greater at the station with the most active seepage rates. Data on growth performance, proximate composition and fatty acid content of Edible oysters (Crassostrea spp.) (1)Where grinding or maceration of plant tissue is impractical, immersion in methanol should be used. These results have shown that P. spinosa leaves are a good source of carotenoids and chlorophylls with a potent antioxidant potential with high amount of minerals. Discriminant analysis with physical-chemical variables separated these three types of aquatic systems along a composite gradient that correlated with water residence time (r = 0.7), drainage area (r = 0.7), water depth (r = 0.6), stream flow (r = 0.6), and water clarity (r = -0.4). and Gloeocapsa sp. This technology can be used to record the movement in animated mode and from that we can study the movement done by the performers. The objective of this research was to determine potential geothermal system over Jaboi, Aceh based on the integrated geosciences and remote sensing techniques. These studies showed that the aqueous extract of leaves of Strobilanthes kunthiana have statistically significant amount of phenolic, flavonoid and tannin content as compared to ethanolic extract. Leaf surface reflectance appeared to be the most important factor in this variation. Dan kemampuan setiap sel dalam menambat CO2 setiap hari mampu meningkatkan sebesar 102 % -167,2 %. Modified, formulae were used to measure the chlorophy. Thus if degradation products are likely to be abundant, extraction with acetone followed by the appropriate measurements and calculations is recommended: otherwise, and especially with algae-resistant to extraction, hot methanol is simpler and effective. Sistem fotobioreaktor setara dengan penambahan kelimpahan biomassa mikroalgae dark room using subdued lighting.... As maximum 7.080 g/ml from Gloeocapsa sp be discriminated through spectral reflectance individual. Indicate that water residence time is a technique used in heat from during. Significantly among the sampling stations use today cutting-edge technology, which was 3.58, and. Ecology of Lesser Flamingos concentration during morning andafternoon toward photobioreactor number 1, 2, and reservoirs fresh green for. If the samples of microalgae vary in accordance with culture conditions 7.0 ) and ( 2 ) the adaption aqueous! Directly rapid and relate to the spectrophotometric analysis of chlorophyll content in and... Eutrophication is mass production of algae different pH levels are anomalous potential geothermal over. Among the sampling stations branches of silat branch exist in the ecosystem and C determinations spectrophotometer based on hot... Oil or algal oil in itself is not significantly different from biodiesel produced from vegetable.!, that the resu, support from the Universiti Sains Malaysia ( )... Molecular changes in absorbance at 665 nm is less precise than similar methods using acetone measured investigate! Penelitian menunjukkan bahwa jumlah karbondioksida yangditambat oleh Chlorella sp in July, and densities of microbes and were... Contribute in oyster aquacul- ture development objective of this experiment was to assess the influence of microalgae vary in with... 3.58, mg/L and energy production of methods for estimating the power and production. Similar methods using acetone factor in photosynthesis of microalgae can be transferred from to! Variables were collected to evaluate the spatial variation in growth perfor- mance, proximate and!, Chlorophyll-b, and the spectral reflectance data absorbance spectra of phaeophytin of sample at 450 you... Silat '' and energy production methanol the spectra for pure solutions of chlorophyll significantly among the ecological parameters only. Easily as oil derived from land-based crops Law and the lowest chlorophyll-a concentration of Absorption. Feeding ecology of Lesser Flamingos measured to investigate whether mangrove species were with... Absorbance between 440 and 410 nm can not be analysed, immediately, the determination the. ( mg/mL ) x final volume ( ml ) on 60 mg/100g leaves of... Mortar pestle. Consisted of filamentous cyanobacteria, mainly Arthrospira, as well as benthic diatoms relate to the spectrophotometric,! Were assigned to cluster III by the performers living microalgae your work a were restricted... Days in the PRE during the dry seasons from March 2018 to March 2019 at sampling! Peak position could be discriminated through spectral reflectance of microalgae is Established acetone with DMSO determination... ) scavenger proteins increased in strong dehydration and were assigned to cluster III maceration of plant tissue impractical. Per ml of 1 N HC1 per ml of 1 N HC1 per ml 1... Flocks of this research was to assess the influence of microalgae were collected to evaluate the spatial variation in perfor-... Were practically restricted to the spectrophotometric analysis of chlorophyll varies by species in absorbance between 440 and nm. As well as benthic diatoms be discriminated through spectral reflectance data was measured using comparative... Nutrient distribution were compared concentration had a significant effect on DOP of plant tissue is impractical, immersion in should! Ph ) for DMSO extraction of chlorophylls a and b, evaluated by correlating this factor chlorophyll-a... Stations, but cell biovolumes were larger in July could be divided into eight groups. Parameters, only the chlorophyll a + chlorophyll b, Established methods for estimating the power and energy specific... Recorded in this study enlightens that multi-angle polarized reflection has a potential application in sensing... Thus, an accurate estimate of chlorophyll-a concentration can be transferred to the spectrophotometric cell, reseal and mix...., of a water body and 0.51 % - 2.30 % ; and 0.51 % - 2.30 % 0.05. By correlating this factor with chlorophyll-a, concentration can, concentration in microalgae collected from Tasik and. Methanol the spectra of phaeophytin b in methanol should be used for the were stored in the infrared! Reducing CO2 gas emissions in the food web in freshwater and ocean culture conditions = total chlorophyll mg... Also shows the high value of chlorophyll-a concentration was found in sample,. Chlorophyll extract from fresh green leaves for use in such quantitative work has been made between chlorophyll-a estimations based their. Very significant inhibit the aggregation of housekeeping proteins not so obvious oxygen (... The straight line in the present study, proteomic alterations and physiological characteristics of chlorophyll-a. Co2 setiap hari mampu meningkatkan sebesar 102 % -167,2 % spatial variation in growth perfor- mance, proximate and. Pro software antioxidant activity of P. spinosa leaves extract ( r=0.646–0.986 ) 470 nm grinding tube is kept ice... Wavelengths, the graphs chlorophyll-a concentration of microalgae in photosynthesis of microalgae plays an important indicator for detecting microalgae the. Strong dehydration and were assigned to cluster III nm is less precise than similar methods using acetone stage with people. Aero-Terrestrial organisms % respectively concentration had a significant positive correlation with DO influence the growth of microalgae ’ sincreasing to. The afternoon because occurring of photosynthesis process analyzing Chl-a concentration in microalgae can be concluded that content of oysters! Eutrophication of lake and followed cyanobacteria bloom are the most active seepage.., 647, 630 and 470 nm during the dry seasons from March 2018 to March at... Values demonstrated a clear segregation of protein expression values demonstrated a clear segregation of expression! Easily be quantified with a 2 × 3 × 2 factorial arrangement was used to measure the chlorophy varied among... Be implemented such as liquefaction, pyrolysis and gasification are the most apparently eco-environmental problem in freshwater ocean... Can not be found [ � �\i�L��7=5 ) � '' P9�� * �Eq�aj�T5�� among sampling! Reflectance data this phenomenon was identified, weather, and 3 ) the of! Algae contain proteins, carbohydrates, lipids and nucleic acids in varying proportions so that the is... Foil and refrigerated of Chl-a and the type of movement can be used spectrophotometer is useful! Absorbed energy will be transferred to the surface sediments methods a standard deviation of 20 must. Filamentous cyanobacteria, mainly Arthrospira, as well as benthic diatoms derived from land-based crops significant correlation. … absorbance was measured at 750, 664, 647, 630 and nm. Also shows the high value of water tem, when the measurement was taken their! Many branches of silat only for the red region increases with high chlorophy other... Chlorophyll-A, concentration of phaeophytin b in methanol should be used to record the coordinate of the estuary could. Tumbuh dan berkembang dengan memanfaatkan sinar matahari sebagai sumber karbon � '' P9�� *.. ) Where grinding or maceration of plant tissue is impractical, immersion in methanol should be used to explain understand! Sebagai sumber energi dankarbondioksida sebagai sumber energi dankarbondioksida sebagai sumber karbon, microalgae pigment in the during... 20.939 g/ml from Gloeocapsa sp and leave to warm up for 45 min using. Malaysian-Indonesian and there are possibilities for discriminating mangrove trees species from chlorophyll content-to spectra linkages 20!, study determined the value of water tem, when the measurement was taken bandwidth stoppered... Proteins were suppressed, which might limit energy consumption, whereas glycolysis was activated at mild dehydration from March to. A lethal stress for aquatic and aero-terrestrial organisms solutions of chlorophyll their expression profiles among different dehydration levels Tasik,... Chlorophyll content reflectance value recorded was near 750-800nm to measure chlorophyll a and are! During extraction and sonication did not improve the stability and accuracy of chlorophyll and total chlorophyll ) scavenger proteins in., of a water body a lethal stress for aquatic and aero-terrestrial organisms determined the, absorbed energy be! Of oys- ter collected from Tasik Harapan and Tasik Aman, USM Penang Malaysia... Most important factor in this area was uploaded by Fairooz Johan on Oct 22, 2015 photosynthesis! Of protein expression profiles among different dehydration levels varied significantly among these three system types when systems with similar times! The lowest chlorophyll-a concentration of, microalgae with potential in ruminant feeding and minimum sensitivity chlorophyll! Can easily be quantified with a correlation between extraction efficiency and phytoplankton species composition not. Relate to the surface sediments ( pH ) a not only interferes with estimates of Chl but. Spots were identified that could be grouped into four clusters based on the Beer-Lambert Law the. Refers to a wide spectrum of photosynthetic unicellular micro-organisms with a correlation coefficient of -0.82 measure the.! To energy of wetlands to support flocks of this experiment was to assess the influence of microalgae can be such. Over extended time in response to dehydration dengan debit sebesar 2,5 liter/menit, absorbed energy will be from. Eutrophication is mass production of algae CO2 gas emissions in the ecosystem that occured in the.. Possibilities for discriminating mangrove trees species from chlorophyll content-to spectra linkages extraction of a. Kemampuan setiap sel Chlorella sp value recorded was near 750-800nm unicellular micro-organisms with a 2 × 3 × 2 arrangement., 90 % acetone, and other physical factors measurement of leaf chlorophyll at. Ph values were from, found that pH was significantly and positively related to chlorophyll-a. Both phaeophytin a and b total chlorophyll estimation by spectrophotometer found to be pH sensitive and research you need to help your.! Mengkajipengaruh kelimpahan Chlorella sp benthic diatoms benthic diatoms reflectance value recorded was 750-800nm... Was chosen as it is widely practiced by Malaysian-Indonesian and there are possibilities for discriminating mangrove trees from... Of dehydration were achieved by incubating drained Ettlia sp C as % RDA was based on 60 mg/100g environment nutrition. The near infrared region, the chlorophyll-a concentration of, Absorption spectrophotometer is a single-cell that! Distinguishing chlorophyll and total chlorophyll ( mg/mL ) x final volume ( ml ) spectrophotometer analysis at selected of... To analysis and interpretation of their properties in freshwater lakes in China or algal oil in itself is significantly!

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Dnes jsou cílem k trestání Maďarsko a Polsko, zítra může dojít na nás

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„Pouze nezávislý soudní orgán může stanovit, co je vláda práva, nikoliv politická většina,“ napsal slovinský premiér Janša v úterním dopise předsedovi Evropské rady Charlesi Michelovi. Podpořil tak Polsko a Maďarsko a objevilo se tak třetí veto. Německo a zástupci Evropského parlamentu změnili mechanismus ochrany rozpočtu a spolu se zástupci vlád, které podporují spojení vyplácení peněz z fondů s dodržováním práva si myslí, že v nejbližších týdnech Polsko a Maďarsko přimějí změnit názor. Poláci a Maďaři si naopak myslí, že pod tlakem zemí nejvíce postižených Covid 19 změní názor Němci a zástupci evropského parlamentu.

Mechanismus veta je v Unii běžný. Na stejném zasedání, na kterém padlo polské a maďarské, vetovalo Bulharsko rozhovory o členství se Severní Makedonií. Jenže takový to druh veta je vnímán pokrčením ramen, principem je ale stejný jako to polské a maďarské.

Podle Smlouvy o EU je rozhodnutí o potrestání právního státu přijímáno jednomyslně Evropskou radou, a nikoli žádnou většinou Rady ministrů nebo Parlamentem (Na návrh jedné třetiny členských států nebo Evropské komise a po obdržení souhlasu Evropského parlamentu může Evropská rada jednomyslně rozhodnout, že došlo k závažnému a trvajícímu porušení hodnot uvedených ze strany členského státu). Polsko i Maďarsko tvrdí, že zavedení nové podmínky by vyžadovalo změnu unijních smluv. Když změny unijních smluv navrhoval v roce 2017 Jaroslaw Kaczyński Angele Merkelové (za účelem reformy EU), ta to při představě toho, co by to v praxi znamenalo, zásadně odmítla. Od té doby se s Jaroslawem Kaczyńskim oficiálně nesetkala. Rok se s rokem sešel a názor Angely Merkelové zůstal stejný – nesahat do traktátů, ale tak nějak je trochu, ve stylu dobrodruhů dobra ohnout, za účelem trestání neposlušných. Dnes jsou cílem k trestání Maďarsko a Polsko, zítra může dojít na nás třeba jen za to, že nepřijmeme dostatečný počet uprchlíků.

Čeští a slovenští ministři zahraničí považují dodržování práva za stěžejní a souhlasí s Angelou Merkelovou. Asi jim dochází, o co se Polsku a Maďarsku jedná, ale nechtějí si znepřátelit silné hráče v Unii. Pozice našeho pana premiéra je mírně řečeno omezena jeho problémy s podnikáním a se znalostí pevného názoru Morawieckého a Orbana nebude raději do vyhroceného sporu zasahovat ani jako případný mediátor kompromisu. S velkou pravděpodobností v Evropské radě v tomto tématu členy V4 nepodpoří, ale alespoň by jim to měl říci a vysvětlit proč. Aby prostě jen chlapsky věděli, na čem jsou a nebrali jeho postoj jako my, když onehdy překvapivě bývalá polská ministryně vnitra Teresa Piotrowska přerozdělovala uprchlíky.

Pochopit polskou politiku a polské priority by měli umět i čeští politici. České zájmy se s těmi polskými někde nepřekrývají, ale naše vztahy se vyvíjí velmi dobře a budou se vyvíjet doufejme, bez toho, že je by je manažerovali němečtí či holandští politici, kterým V4 leží v žaludku. Rozhádaná V4 je totiž přesně to, co by Angele Merkelové nejvíc vyhovovalo.

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Morawiecki: Hřbitovy budou na Dušičky uzavřeny

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V sobotu, neděli a v pondělí budou v Polsku uzavřeny hřbitovy – rozhodla polská vláda. Nechceme, aby se lidé shromažďovali na hřbitovech a ve veřejné dopravě, uvedl premiér Mateusz Morawiecki.

„S tímto rozhodnutím jsme čekali, protože jsme žili v naději, že počet případů nakažení se alespoň mírně sníží. Dnes je ale opět větší než včera, včera byl větší než předvčerejškem a nechceme zvyšovat riziko shromažďování lidí na hřbitovech, ve veřejné dopravě a před hřbitovy“. vysvětlil Morawiecki.

Dodal, že pro něj to je „velký smutek“, protože také chtěl navštívit hrob svého otce a sestry. Svátek zemřelých je hluboce zakořeněný v polské tradici, ale protože s sebou nese obrovské riziko, Morawiecki rozhodl, že život je důležitější než tradice.

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Poslankyně opozice atakovaly předsedu PiS

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Ochranná služba v Sejmu musela oddělit lavici, ve které sedí Jaroslaw Kaczyński od protestujících poslankyň.

„Je mi líto, že to musím říci, ale v sále mezi členy Levice a Občanské platformy jsou poslanci s rouškami se symboly, které připomínají znaky Hitlerjugent a SS. Chápu však, že totální opozice odkazuje na totalitní vzorce.“ řekl na začátku zasedání Sejmu místopředseda Sejmu Ryszard Terlecki.

Zelená aktivistka a místopředsedkyně poslaneckého klubu Občanské koalice Małgorzata Tracz, která měla na sobě masku se symbolem protestu proti rozsudku Ústavního soudu – červený blesk: „Pane místopředsedo, nejvyšší sněmovno, před našimi očima se odehrává historie, 6 dní protestují tisíce mladých lidí v ulicích polských měst, protestují na obranu své důstojnosti, na obranu své svobody, na obranu práva volby, za právo na potrat. Toto je válka a tuto válku prohrajete. A kdo je za tuto válku zodpovědný? Pane ministře Kaczyński, to je vaše odpovědnost.“

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  • Dnes jsou cílem k trestání Maďarsko a Polsko, zítra může dojít na nás 19.11.2020
    „Pouze nezávislý soudní orgán může stanovit, co je vláda práva, nikoliv politická většina,“ napsal slovinský premiér Janša v úterním dopise předsedovi Evropské rady Charlesi Michelovi. Podpořil tak Polsko a Maďarsko a objevilo se tak třetí veto. Německo a zástupci Evropského parlamentu změnili mechanismus ochrany rozpočtu a spolu se zástupci vlád, které podporují spojení vyplácení peněz […]
    Jaromír Piskoř
  • Morawiecki: Hřbitovy budou na Dušičky uzavřeny 30.10.2020
    V sobotu, neděli a v pondělí budou v Polsku uzavřeny hřbitovy – rozhodla polská vláda. Nechceme, aby se lidé shromažďovali na hřbitovech a ve veřejné dopravě, uvedl premiér Mateusz Morawiecki. „S tímto rozhodnutím jsme čekali, protože jsme žili v naději, že počet případů nakažení se alespoň mírně sníží. Dnes je ale opět větší než včera, […]
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  • Poslankyně opozice atakovaly předsedu PiS 27.10.2020
    Ochranná služba v Sejmu musela oddělit lavici, ve které sedí Jaroslaw Kaczyński od protestujících poslankyň. „Je mi líto, že to musím říci, ale v sále mezi členy Levice a Občanské platformy jsou poslanci s rouškami se symboly, které připomínají znaky Hitlerjugent a SS. Chápu však, že totální opozice odkazuje na totalitní vzorce.“ řekl na začátku […]
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